In a small way, yes it did.
However, the deleterious effects of this incident would have been almost entirely isolated to the southern plantation colonies of Virginia, the Carolinas, and Georgia. Not only are those only four of the 13 colonies, but they happen to have been some of the rebelling colonies in which Royalists (colonists against the revolution and for staying part of England) were the strongest. For a while during the war, Georgia and South Carolina were under Royalist administration and were fighting on the side of the British.
Due to the necessity of correspondence in the trans-Atlantic communications involved in the dispute, the slide of the American colonies from loyalty into open rebellion is probably one of the better documented eras in history.
I recently got a compilation of such correspondence. From what I've seen, probably the best written and most comprehensive list of colonial grievances is the one laid out by Benjamin Franklin in late 1773, in his sarcastic piece Rules by Which a Great Empire May Be Reduced To a Small One. Note that there is a mention in there of "Stock-Jobbers", but it is one of nearly a hundred things. Being 1773, it misses the last 3 years of activity before the final breach, but if you imagine England doubling down on the behavior mentioned rather than retarding it, you'll get the idea.
There appears to have been a fundamental dispute of power between the British Parliament and the colonies. The colonists felt like they should be free British citizens, with all the attendant rights and privileges they would enjoy if they resided on the home island. One thing I'm finding is that the concepts of man's natural freedom expressed in the USA's founding documents were not original there. Rather they are just a concise expression of the rights free men in England already enjoyed. So the issue wasn't that Americans had a radically different conception of a citizen's rights than existed in England, but rather that they felt they ought to still possess them even outside the borders of that island.
Parliament's position seems to have been that the colonies, including all the property held within, were simply a Crown possession. As the official Government of England, they could make any law they liked governing the property and people inside an English colony. Any loyal British citizen must comply, and any failure to do so must be punished or it would just encourage such behavior worldwide.
So it is certainly fair to say that "stock-jobbers" like the tobacco lords had their hand in the revolt. However, they were just one of a cast of thousands. The root issue was a dispute over who had the right to legislate over the colonists. Nearly all the other problems flowed out of that.