What language(s) were considered the primary language for the Holy Roman Empire? Were there many different languages spoken due to the many different regions?
The Holy Roman Empire (of the German Nations) was a multi-ethnic, multi-cultural, and multi-lingual coalition from its (unofficial) founding by Charlemagne in the 9th century AD. The German Empire would be a better term in fact, as it was founded and typically ruled by Germanic peoples. (Charlemagne himself was a Frank.) As Voltaire once perceptively quipped, the Holy Roman Empire was "neither holy, nor Roman, nor an empire". (Essai sur l'histoire générale et sur les mœurs et l'esprit des nations, Chapter 70)
Given that the boundaries of the empire were constantly changing over its almost thousand-year history (and were rarely if ever officially defined), the cultures and languages subsumed were constantly in flux too. Undoubtedly, German (or the predominant dialect thereof) was the de-facto official language. Latin was also for official matters of state/ceremonies, especially during the Medieval period, given this modern empire's desire to ape the glory of Ancient Rome, not to mention the ubiquity of the Church in Medieval life.
A map of the Holy Roman territories cerca 1600 hints well at what languages were commonly spoken by the populace in various regions.
These would have included the following:
Undoubtedly I have missed out a few, but the above list should cover the predominant languages within the (stable) boundaries of the Holy Roman Empire, noting where appropiate the diversity of dialects of some languages that was extremely prevalent during the medieval and early modern periods of history.
Medieval Latin (an evolution of the Classical Latin of Ancient Rome) was indeed the official language of the Holy Roman Empire for most of its history. It was in fact only changed to Standard German during the reign of Joseph II (late 18th century.) What is interesting to note, especially in the High Medieval period where the Holy Roman Empire encompassed much land and many cultures, is that Medieval Latin was the only common language between the educated (especially ecclesiastical) folk in all of its member states. It was perhaps the second great period in which Latin was the "Lingua Franca" of its day, supplanted by Standard German (even in regions such as Slavic Bohemia) by the Early Modern period.
Please feel free to add/improve on references, especially secondary ones. Some of the information in this post is admittedly from old history lessons of mine/sources I have forgotten.
Mainly German, but a lot of literature wasn't vernacular (in your language) until after the printing press, so Latin was used to read books (if you were educated). Italian was also used in the southern part of the HRE.
(Pulled from A History of Western Society by John McKay)
In a decree "Golden Bull of 1356" which sets the election system of the Holy Roman Emperor, there was written that the elector sons should speak the three imperial languages - German, Italian and Czech.
I'm unable to find a reference to an official language of the empire, but according to Wikipedia, the languages that would have been spoken within its borders were numerous, and would include the following:
taken from here
As for which one would have been the language of business, I would say Latin during its inception and pre-Renaissance existence, the Germanic languages as the independent states of the era came into their own during the latter half of the empire time, and Italian as well during that time, but fading as Italian influence waned during the last two hundred or so years.